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Calcium transport study of sf 9 lepidopteran cells and bull frog sympathetic ganglion cells

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The free Biology research paper Calcium Transport essay presented on this page should not be viewed as a sample of our on-line writing service. It was found that the int Spodoptera frugiperda clone 9 SF-9 cells are a cultured insect cell line derived from the butterfly ovarian tissue. SF-9 cells are used by molecular biologists for the studies of gene expression and protein processing Luckow and Summers, 1988.

Moreover, the calcium extrusion rates of both cell types with no Na2VO4 added seemed to the same. However, due to insufficient data, the effects of Na2V After obtaining these basic parameters, many questions raised such as how does the SF-9 cells extrude their calcium and why the Na2VO4 affected the calcium efflux for the SF-9 cells but not the BSG cells? It was then sonicated for 10 minutes. It was then kept frozen until the day of the experiment.

A study of calcium transport of sf 9 lepidopteran cells in bull frogs

The above solutions were adjusted to pH 7. The cells were plated on custom made 3. A SF-9 cells non-transfected were cultured as described by Summers and Smith 1987.

  • It was then kept frozen until the day of the experiment;
  • It was then kept frozen until the day of the experiment.

Intracellular calcium measurements Fura-2 is a fluorescence indicator of calcium that is used to determine the free intracellular calcium concentration. The fluorescence microscope unit consisted of a 75 W Xenon arc lamp and a Zeiss inverted microscope with a Zeiss Neofluor 63X objective.

A study of calcium transport of sf 9 lepidopteran cells in bull frogs

In addition, a pipette was placed close to the sample cells within 5mm for perfusion. Emitted fluorescence signal was detected by the photomultiplier tube PMT and recorded via a NEC 286 microc The experimental methods of calcium measurements used in the experiments were similar to the one described by Schwartz et al.

In brief, intracellular free calcium concentration can be determined through the following formula Grynkiewicz et al. When the intracellular calcium level was stabilized, it was switched to 2-bromo-A23187 to raise Statistical analysis was performed with using The Student Edition of Minitab release 8 Minitab Inc.

  1. Dna processing is not required for atm-mediated telomere damage response after trf2 deletion cells unexpectedly to study these issues because they provide. A study of calcium transport of sf 9 lepidopteran cells in bull frogs The dictionary of virology this page intentionally left blank the dictionary of virology fourth edition brian wj mahy division of emerging infections and.
  2. Phm499 research project calcium transport study of sf-9 lepidopteran cells sympathetic ganglion cells bsg and the sf-9 basic biophysiology of these cells.
  3. Calcium transport study of sf-9 lepidopteran cells and bull frog sympathetic ganglion cellsabstract the intracellular calcium level and the calcium efflux of the bull.
  4. Effects of intracellular calcium injection on steady state membrane currents in isolated single ventricular cells microcalorimetric study of the biological.

Results It was found that the intracellular calcium concentration in the SF-9 cells was 44. The calcium concentration in the BSG cells was found to be 58. Student's t test did not indicate a significant The rates of active transport of calcium out of the cells following 0CaNRS were also calculated.