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Chemical and physical process of digestion lab chart1

The fact that some of the end products of fat digestion fatty acids are organic acids that decrease the pH provides an easy way to recognize that digestion is ongoing or completed. You will be using a pH indicator called litmus blue to follow these changes; it changes from blue to pink as the test tube contents become acid.

  1. Quizlet provides chemical and physical processes of digestion activities chemical and physical process of digestion lab 3 chemical and physical processes of. From the general supply area, obtain 9 test tubes and a test tube rack, plus one dropper bottle of each of the solutions in supply area 3.
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  3. Explain the role of bile in the digestive process chart 1 salivary amylase digestion of starch chemical and physical processes of digestion. Physical and chemical changes in and physical changes occur in the digestion process and support their digestion, physical change, chemical change.

From the general supply area, obtain 9 test tubes and a test tube rack, plus one dropper bottle of each of the solutions in supply area 3. Although bile, a secretory product of the liver, is not an enzyme, it is important to fat digestion because of its emulsifying action the physical breakdown of larger particles into smaller ones on fats.

Chemical and physical process of digestion lab chart1

Emulsified fats provide a larger surface area for enzymatic activity. To demonstrate the action of bile on fats, prepare two test tubes and mark them 1E and 2E.

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  • Remove the Parafilm and place all tubes in a rack in the appropriate water bath for approximately 1 hour.

To tube 1E add 10 drops of water and 2 drops of vegetable oil. To tube 2E add 10 drops of water, 2 drops of vegetable oil, and a pinch of bile salts. Cover each tube with a small square of Parafilm, shake vigorously, and allow the tubes to stand at room temperature.

After 10-15 minutes, observe both tubes.

If emulsification has not occurred, the oil will be floating on the surface of the water. If emulsification has occurred, the fat droplets will be suspended throughout the water, forming an emulsion.

In which tube has emulsification occurred? Two students should prepare the controls 1L, and 2L,while the other two students in the group set up the experimental samples 3L to 5L, 4B, and 5B as indicated in Chart 3.

  1. A pinch of bile salts should be placed in tubes 4B and 5B.
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  3. Chemical and physical processes of digestion fall 2008 assignment due november 14, 2008 be sure you understand the concepts before starting the lab exercise. Review sheet exercise 8 chemical and physical processes of digestion name.

Mark each tube with a wax pencil and load the tubes using 5 drops of each indicated solution. A pinch of bile salts should be placed in tubes 4B and 5B. Cover each tube with Parafilm and shake to mix the contents of the tube.

Chemical and physical process of digestion lab chart1

Boil tube 3L for 4 minutes prior to starting the incubation. Remove the Parafilm and place all tubes in a rack in the appropriate water bath for approximately 1 hour. The basis of this assay is a pH change that is detected by a litmus powder indicator.

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Alkaline or neutral solutions containing litmus are blue but will turn reddish in the presence of acid. Since fats are digested to fatty acids organic acids during hydrolysis, they lower the pH of the sample they are in.

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  • To prepare a color control, add 0.

Litmus cream fresh cream providing the fat substrate to which litmus powder was added will turn from a bluish color to pink if the solution is acid. Because the effect of hydrolysis is directly seen, additional assay reagents are not necessary. To prepare a color control, add 0. Record the color of the tubes in Chart 3 and on the chalkboard.